Part:BBa_K118024

dxs+LIMS1+appY

dxs, from Escherichia coli JM109, encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids, and appY, also from Escherichia coli JM109, encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of dxs and appYhas been reported to increase yields of carotenoids and other terpenoids (Kang, M.J., Lee, Y.M., Yoon, S.H., Kim, J.H., Ock, S.W., Jung, K.H., Shin, Y.C., Keasling, J.D., and Kim, S.W. 2005. Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method. Biotechnology and Bioengineering 91, 636-642). LIMS1, from Citrus limon, encodes limonene synthase, which produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway)(Lucker, J., El Tamer, M.K., Schwab, W., Verstappen, F.W.A., van der Plas, L.H.W., Bouwmeester, H.J., and Verhoeven, H.H. 2002. Monoterpene biosynthesis in lemon (Citrus limon). European Journal of Biochemistry 269, 3160-3171). (+)-Limonene is a component of lemon scent. (All three genes are individually preceded by a ribosome binding site.)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 746
  Illegal SapI.rc site found at 2006
  

Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 9.8 ± 5.8%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.