Translational_Unit

Part:BBa_K515002

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 16:39, 21 September 2011 by Nikkikapp (Talk | contribs)

PA2652

Description

Part of BioBrick BBa_K515102, includes optimised insulator, RBS and coding sequence for a malate-responsive chemoreceptor PA2652 from Pseudomonas aeruginosa PA01. Th insulator allows the strength of the RBS to remain the same even when the promoters for this BioBrick are changed using PCR, with the insulator as the start for primers. The translation initiation rate for the RBS sequence is 42010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 675
    Illegal NgoMIV site found at 777
    Illegal AgeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 984


[edit]
Categories
//cds/membrane/receptor
Parameters
chassisE. coli DH5α
device_typeChemotaxis receptor
ligandsMalate
originP. aeruginosa
resistanceChloramphenicol