Difference between revisions of "Part:BBa K323132:Design"

 
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<partinfo>BBa_K323132 short</partinfo>
 
<partinfo>BBa_K323132 short</partinfo>
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===Design Notes===
 
===Design Notes===
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[[Part:BBa_K323087]] was ligated into a vector containing [[Part:BBa_K323086]] as a back insert. [[Part:BBa_K323131]] was later ligated into the resulting BioBrick as a back insert. The fragment containing the latter three BioBrick parts was then ligated into the ''Bsa''I cloning site of [[Part:BBa_K323110]].
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===Source===
 
===Source===

Revision as of 03:54, 27 October 2010

VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3078
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 4320
    Illegal SapI.rc site found at 4395


Design Notes

Part:BBa_K323087 was ligated into a vector containing Part:BBa_K323086 as a back insert. Part:BBa_K323131 was later ligated into the resulting BioBrick as a back insert. The fragment containing the latter three BioBrick parts was then ligated into the BsaI cloning site of Part:BBa_K323110.

Source

a

References