Difference between revisions of "Part:BBa K323089:Design"
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[[Part:BBa_I732019]] was ligated into a vector containing [[Part:BBa_B0015]] as a front insert. [[Part:BBa_K323110]] was then ligated with the resulting construct as a back insert. | [[Part:BBa_I732019]] was ligated into a vector containing [[Part:BBa_B0015]] as a front insert. [[Part:BBa_K323110]] was then ligated with the resulting construct as a back insert. | ||
− | ===Source | + | ===Source== |
− | + | Part:BBa_B0015 and Part:BBa_I732019 were taken from the Registry of parts, and Part:BBa_K323110 was previously constructed by our team (for more information see [[Part:BBa_K323110:Design]]) | |
===References=== | ===References=== |
Revision as of 18:24, 26 October 2010
In vivo testing device for protein-DNA binding: part 2 (lacZ_DTER_pBAD_BsaI_DTER)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4441
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4381
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4216
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4496
Illegal BsaI.rc site found at 4472
Illegal SapI site found at 4198
Design Notes
Part:BBa_I732019 was ligated into a vector containing Part:BBa_B0015 as a front insert. Part:BBa_K323110 was then ligated with the resulting construct as a back insert.
=Source
Part:BBa_B0015 and Part:BBa_I732019 were taken from the Registry of parts, and Part:BBa_K323110 was previously constructed by our team (for more information see Part:BBa_K323110:Design)