Difference between revisions of "Part:BBa K299008"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K299008 short</partinfo> | <partinfo>BBa_K299008 short</partinfo> | ||
| − | + | {{RBSMeasurement|name=BBa_B0033|kind=community|strength=0.35%}} | |
Latest revision as of 22:35, 25 October 2010
RBS measurement device J23100 B0033 GFP
This part is licensed under
Creative BioCommons
This device was used to determine BBa_B0033 strength relative to BBa_B0034.
Authors
Clonning:
- Ania Puławska
- Ania Safray
- Milena Bażlekowa
The lab work was supervised by:
- Jarek Pankowski
- Ania Olchowik
- Kasia Grześ
Flow cytometer measurements done by Cherry Moreno
Construct design
The device consists of BBa_J23100 promoter followed by BBa_B0033 community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein.
Measurements
Using this construct we have determined that BBa_B0033 strength is 0.35% relative to BBa_B0034
All our measurement results are summarized on the chart below:
For more info please look at Team Warsaw 2010 [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS measurement page].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 704