Difference between revisions of "Part:BBa K389016:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K389016 short</partinfo>
 
<partinfo>BBa_K389016 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
this is only for measuring the natural virA/G system
+
* The ''virA'' gene is under the control a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)
 +
* double terminator (forward) to keep expression of mRFP by the constitutive promoter low
 +
** double terminator <partinfo>BBa_B0017</partinfo> instead of <partinfo>BBa_B0015</partinfo> because of problems mentioned with <partinfo>BBa_B0012</partinfo>
 +
* mRFP gene to show the activity of the ''vir'' promoter
 +
* this BioBrick is for measuring the natural virA/G system
  
  
Line 13: Line 16:
 
===Source===
 
===Source===
  
−
''A. tumefaciens'' C58 TI-plasmid, parts.igem, Mr. Gene
+
* ''virA'' from ''A. tumefaciens'' C58 (<partinfo>K389001</partinfo>)
 +
* ''virG'' gene synthesized by Mr. Gene (<partinfo>BBa_K389002</partinfo>)
 +
* ''vir'' promoter from ''A. tumefaciens'' C58 (<partinfo>BBa_K389003</partinfo>)
 +
* constitutive promoter, RBS, double terminator and mRFP from parts.igem (<partinfo>BBa_J23110</partinfo>, <partinfo>B0034</partinfo>, <partinfo>BBa_B0017</partinfo>, <partinfo>E1010</partinfo>)
  
 
===References===
 
===References===

Revision as of 13:32, 7 October 2010

VirA/G reporter device mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4260
    Illegal AgeI site found at 4372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768


Design Notes

  • The virA gene is under the control a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)
  • double terminator (forward) to keep expression of mRFP by the constitutive promoter low
  • mRFP gene to show the activity of the vir promoter
  • this BioBrick is for measuring the natural virA/G system


Source

References