Difference between revisions of "Featured Parts:Light Sensor"

Revision as of 21:12, 4 May 2006

System overview

From Levskyaya et al.

We have designed a bacterial system that is switched between different states by red light. The system consists of a synthetic sensor kinase that allows a lawn of bacteria to function as a biological film, such that the projection of a pattern of light on to the bacteria produces a high-definition (about 100 megapixels per square inch), two-dimensional chemical image.

Sample photos

Here are a selection of sample coliroid taken with the bacterial photography system.

http://openwetware.org/images/9/9a/Macintosh_HD-Users-nkuldell-Desktop-bacterialselfportrait.jpg	http://openwetware.org/images/8/83/UT_HelloWorld.jpg	http://openwetware.org/images/thumb/d/d1/ColiroidEllington.png/200px-ColiroidEllington.png	http://openwetware.org/images/6/68/ColiroidFSM.jpg
[http://openwetware.org/wiki/Jeff_Tabor Jeff Tabor] holding a coliroid. Photo credit: Marsha Miller, University of Texas at Austin. Image courtesy of UT/UCSF.	Hello World coliroid published in Levskaya et al., Nature, 2005.	This is a coliroid portait of Andy Ellington. You can compare it with the [http://www.icmb.utexas.edu/images/faculty/ellington.jpg real Andy]. Image courtesy of UT/UCSF.	This is a coliroid of the [http://venganza.org Flying Spaghetti Monster]. Image courtesy of UT/UCSF.

Device implementation

This system consists of two devices.

A light sensor which takes red light as an input and produces PoPS as an output.
A color generator which takes a PoPS signal as input and generate color as an output.

Diagram courtesy of [http://openwetware.org/wiki/Drew_Endy Drew Endy].

Parts implementation

This diagram shows the list of parts that make up each of the two devices.

Diagram courtesy of [http://openwetware.org/wiki/Drew_Endy Drew Endy].

BioBricks parts list

Light sensing parts

Color generating parts

Related parts

BBa_M30109

Contact

System design and implementation by [http://openwetware.org/wiki/User:Levskaya Anselm Levskaya], Aaron Chevalier, [http://openwetware.org/wiki/Jeff_Tabor Jeff Tabor], [http://openwetware.org/wiki/User:LLavery Laura Lavery], Matthew Levy, Eric Davidson, Alexander Scouras, [http://ellingtonlab.org/ Andy Ellington], Ed Marcotte, and [http://www.voigtlab.ucsf.edu/ Chris Voigt].

References

Papers

Engineering Escherichia coli to see light
Nature 24 November 2005 DOI:10.1038/nature04405
A. Levskaya et al.
[http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html URL] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16306980 Pubmed] [http://www.hubmed.org/display.cgi?uids=16306980 Hubmed]

Other

[http://openwetware.org/wiki/BE.109:Systems_engineering MIT Biological Engineering laboratory module] on bacterial photography developed by [http://openwetware.org/wiki/Natalie_Kuldell Natalie Kuldell]
[http://openwetware.org/wiki/LightCannon Instructions] for how to build a "light cannon" for use in bacterial photography.

@@ Line 19: / Line 19: @@
 | This is a '''<font color="brown">coliroid</font>''' of the [http://venganza.org Flying Spaghetti Monster]. Image courtesy of UT/UCSF.
 |}
 ==Device implementation==
+This system consists of two devices.
+#A light sensor which takes red light as an input and produces PoPS as an output.
+#A color generator which takes a PoPS signal as input and generate color as an output.
+[[Image:Coliroiddevices.jpg]]<br>
+Diagram courtesy of [http://openwetware.org/wiki/Drew_Endy Drew Endy].
 ==Parts implementation==
+This diagram shows the list of parts that make up each of the two devices.
+[[Image:Coliroidparts.jpg]]<br>
+Diagram courtesy of [http://openwetware.org/wiki/Drew_Endy Drew Endy].
 ===BioBricks parts list===
-====System parts====
+====Light sensing parts====
 *[https://parts.igem.org/wiki/index.php/Part:BBa_I15008 BBa_I15008]
 *[https://parts.igem.org/wiki/index.php/Part:BBa_I15009 BBa_I15009]
 *[https://parts.igem.org/wiki/index.php/Part:BBa_I15010 BBa_I15010]
+*[https://parts.igem.org/wiki/index.php/Part:BBa_R0082 BBa_R0082]
+====Color generating parts====
+*[https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]
+*[https://parts.igem.org/wiki/index.php/Part:BBa_E0033 BBa_E0033]
+*[https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]
 ====Related parts====
@@ Line 36: / Line 51: @@
 ==Contact==
 *System design and implementation by [http://openwetware.org/wiki/User:Levskaya Anselm Levskaya], Aaron Chevalier, [http://openwetware.org/wiki/Jeff_Tabor Jeff Tabor], [http://openwetware.org/wiki/User:LLavery Laura Lavery], Matthew Levy, Eric Davidson, Alexander Scouras, [http://ellingtonlab.org/ Andy Ellington], Ed Marcotte, and [http://www.voigtlab.ucsf.edu/ Chris Voigt].
-*This summary was assembled by [http://openwetware.org/wiki/Reshma_Shetty Reshma Shetty].
 ==References==
 ===Papers===
-<biblio>
+#'''Engineering Escherichia coli to see light'''<br> ''Nature'' 24 November 2005 DOI:10.1038/nature04405<br> A. Levskaya ''et al.''<br> [http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html URL] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16306980 Pubmed] [http://www.hubmed.org/display.cgi?uids=16306980 Hubmed]
-#Levskaya-Nature-2005 pmid=16306980
-</biblio>
 ===Other===
 *[http://openwetware.org/wiki/BE.109:Systems_engineering MIT Biological Engineering laboratory module] on bacterial photography developed by [http://openwetware.org/wiki/Natalie_Kuldell Natalie Kuldell]
 *[http://openwetware.org/wiki/LightCannon Instructions] for how to build a "light cannon" for use in bacterial photography.