Difference between revisions of "Part:BBa K5117044"
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This part serves as transcriptional unit composed of: | This part serves as transcriptional unit composed of: | ||
− | + | <ul> | |
+ | <li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021)</a></html></li> | ||
− | + | <li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html></li> | |
- gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for <i>Bacillus subtilis</i> (Xi <i>et al.</i> 2021) without any signal peptide, | - gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for <i>Bacillus subtilis</i> (Xi <i>et al.</i> 2021) without any signal peptide, | ||
− | addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA (BBa_K5117032) | + | <div style="text-indent: 1px;">addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA <html><a href="https://parts.igem.org/Part:BBa_K5117032"(BBa_K5117032)</a></html></div> |
− | + | <li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html></li> | |
− | + | <li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html></li> | |
+ | </ul> | ||
+ | <b>Biosafety level:</b> S1 | ||
+ | |||
+ | <b>Target organism:</b> <i>Bacillus subtilis</i> | ||
+ | |||
+ | <b>Main purpose of use:</b> Immobilization of BhrPET on the spore crust of <i>B. subtilis</i> (spore surface display) | ||
+ | |||
+ | <b>Application:</b> Degradation of PET | ||
− | |||
− | |||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K5117044 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5117044 SequenceAndFeatures</partinfo> |
Revision as of 16:19, 1 October 2024
PcotYZ-BsRBS-BhrPET-L1-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
- gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021) without any signal peptide,
addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of BhrPET on the spore crust of B. subtilis (spore surface display)
Application: Degradation of PET
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1009
Illegal AgeI site found at 695
Illegal AgeI site found at 740 - 1000COMPATIBLE WITH RFC[1000]