Coding
Part:BBa_K5117032
Designed by: Jenny Sauermann Group: iGEM24_TU-Dresden (2024-09-28)
BhrPET-L1
gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021) without any signal peptide
Downstream of the coding sequence, a short flexible linker (L1) has been added encoding the amino acids GA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 495
Illegal AgeI site found at 540 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |