Coding

Part:BBa_K5117032

Designed by: Jenny Sauermann   Group: iGEM24_TU-Dresden   (2024-09-28)


BhrPET-L1

gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021) without any signal peptide

Downstream of the coding sequence, a short flexible linker (L1) has been added encoding the amino acids GA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 495
    Illegal AgeI site found at 540
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None