Difference between revisions of "Part:BBa K5205002"

 
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The OmpA (outer membrane protein A) fragment from amino acids 46 to 159 is a segment of the protein that spans part of the extracellular domain in E. coli (Earhart, 2000).  
 
The OmpA (outer membrane protein A) fragment from amino acids 46 to 159 is a segment of the protein that spans part of the extracellular domain in E. coli (Earhart, 2000).  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K5205002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5205002 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K5205002 parameters</partinfo>
 
<partinfo>BBa_K5205002 parameters</partinfo>
 
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===Usage and Biology===
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This partial OmpA is often fused with signal peptides such as Lpp or Non ([https://parts.igem.org/Part:BBa_K5205001 BBa_K5205001], a signal-like sequence) to construct a cell surface display system, as seen in constructs like Lpp-OmpA (BBa_K1694010) (Earhart, 2000) and Non-OmpA [https://parts.igem.org/Part:BBa_K5205003 BBa_K5205003] (Jeiranikhameneh et al., 2017).
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===References===
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Earhart, C. F. (2000). Use of an Lpp-OmpA fusion vehicle for bacterial surface display. Methods Enzymol, 326, 506-516. https://doi.org/10.1016/s0076-6879(00)26072-2
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Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0

Revision as of 02:50, 24 September 2024


Amino acid 46-159 of OmpA, the extracellular fragment of outer membrane protein A from E. coli

The OmpA (outer membrane protein A) fragment from amino acids 46 to 159 is a segment of the protein that spans part of the extracellular domain in E. coli (Earhart, 2000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

This partial OmpA is often fused with signal peptides such as Lpp or Non (BBa_K5205001, a signal-like sequence) to construct a cell surface display system, as seen in constructs like Lpp-OmpA (BBa_K1694010) (Earhart, 2000) and Non-OmpA BBa_K5205003 (Jeiranikhameneh et al., 2017).

References

Earhart, C. F. (2000). Use of an Lpp-OmpA fusion vehicle for bacterial surface display. Methods Enzymol, 326, 506-516. https://doi.org/10.1016/s0076-6879(00)26072-2

Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0