Difference between revisions of "Part:BBa K200018"
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===Usage and Biology===
 
===Usage and Biology===
−
This construct was used by Imperial College's 2009 iGEM team for [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project. It was ligated to [https://parts.igem.org/Part:BBa_J5526 BBa_J5526] to produce the final construct [https://parts.igem.org/wiki/index.php/Part:BBa_K200019 BBa_K200019].
+
This construct was used by Imperial College's 2009 iGEM team for [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project using the following subparts: [https://parts.igem.org/wiki/index.php/Part:BBa_K118011 BBa_K118011], [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034], [https://parts.igem.org/wiki/index.php/Part:BBa_E0040 BBa_E0040] and [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]. It was ligated to [https://parts.igem.org/Part:BBa_J5526 BBa_J5526] to produce the final construct [https://parts.igem.org/wiki/index.php/Part:BBa_K200019 BBa_K200019].
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 21:55, 19 October 2009

pCstA+RBS+GFP+TT

This part contains a GFP (green fluorescent protein) reporter in the functional transcriptional unit. As such, activity of the pCstA promoter can be assessed by the expression of GFP.


Usage and Biology

This construct was used by Imperial College's 2009 iGEM team for [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project using the following subparts: BBa_K118011, BBa_B0034, BBa_E0040 and BBa_B0015. It was ligated to BBa_J5526 to produce the final construct BBa_K200019.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 801