Difference between revisions of "Part:BBa K4115039"
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| − | This composite part is used to detect the response of LuxR [https://parts.igem.org/Part:BBa_C0062 ( | + | This composite part is used to detect the response of LuxR [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)] to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express. |
Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations between E.coil and S.elongatus through quorum sensing signals. | Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations between E.coil and S.elongatus through quorum sensing signals. | ||
==Proof of function== | ==Proof of function== | ||
| + | |||
| + | We encapsulated E. coli with the CP and E. coli with LuxI into alginate gel beads. The E. coli with LuxI secreted the 3OC6 HSL signal molecule, and the E. coli with LuxR received the Signal and regulate the expression of downstream sfGFP, making it express. So we can detect green fluorescence in the latter E. coli under the irradiation of ultraviolet light. | ||
[[File:LuxR regulations between E.coli.jpg|500px|]] | [[File:LuxR regulations between E.coli.jpg|500px|]] | ||
| − | '''Figure 1:''' sfGFP is expressed under the regulation of LuxR between E.coli in a single gel bead. | + | '''Figure 1:''' sfGFP is expressed under the regulation of LuxR between E.coli in a single gel bead. Two parallel groups are given and the above two are control groups. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:24, 3 October 2022
J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
| J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015 | |
|---|---|
| Function | gene regulator |
| Use in | E.coli |
| RFC standard | RFC 10, RFC 10 compatible |
| Backbone | pBBR1 |
| Submitted by | (ShanghaiTech_China) |
This composite part is used to detect the response of LuxR (BBa_C0062) to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (BBa_R0062) to make superfolder GFP (BBa_K4115000) express.
Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations between E.coil and S.elongatus through quorum sensing signals.
Proof of function
We encapsulated E. coli with the CP and E. coli with LuxI into alginate gel beads. The E. coli with LuxI secreted the 3OC6 HSL signal molecule, and the E. coli with LuxR received the Signal and regulate the expression of downstream sfGFP, making it express. So we can detect green fluorescence in the latter E. coli under the irradiation of ultraviolet light.
Figure 1: sfGFP is expressed under the regulation of LuxR between E.coli in a single gel bead. Two parallel groups are given and the above two are control groups.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985