Difference between revisions of "Part:BBa K4115039"

Revision as of 08:30, 3 October 2022

J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015

J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
Function	gene regulator
Use in	E.coli, A.caulinodans ORS571
RFC standard	RFC 10, RFC 10 compatible
Backbone	pBBR1
Submitted by	(ShanghaiTech_China)

This composite part is used to detect the response of LuxR (C0062) to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (R0062) to make superfolder GFP (BBa_K4115000) express.

Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations between E.coil and S.elongatus through quorum sensing signals.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 985

@@ Line 9: / Line 9: @@
 |-
 |'''Use in'''
-|E.coli
+|E.coli, A.caulinodans ORS571
 |-
 |'''RFC standard'''
@@ Line 23: / Line 23: @@
 This composite part is used to detect the response of LuxR [https://parts.igem.org/Part:BBa_C0062 (C0062)] to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express.
-Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications between E.coil and S.elongatus through quorum sensing signals.
+Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations between E.coil and S.elongatus through quorum sensing signals.