Difference between revisions of "Part:BBa K4115039"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4115039 short</partinfo>
 
<partinfo>BBa_K4115039 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
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|-
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|'''Function'''
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|gene regulator
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|-
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|'''Use in'''
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|E.coli
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10], RFC 10 compatible
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|-
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|'''Backbone'''
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|pBBR1<br>
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|-
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|'''Submitted by'''
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|[https://2022.igem.wiki/shanghaitech-china/ (ShanghaiTech_China)]
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|}
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This composite part is used to detect the response of LuxR [https://parts.igem.org/Part:BBa_C0062 (C0062)] to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express.
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Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications between E.coil and S.elongatus through quorum sensing signals.
  
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none
 
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 08:28, 3 October 2022


J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015

J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
Function gene regulator
Use in E.coli
RFC standard RFC 10, RFC 10 compatible
Backbone pBBR1
Submitted by (ShanghaiTech_China)

This composite part is used to detect the response of LuxR (C0062) to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (R0062) to make superfolder GFP (BBa_K4115000) express.

Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications between E.coil and S.elongatus through quorum sensing signals.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985