Difference between revisions of "Part:BBa K3868075"
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Promoter of glucose-6-phosphate isomerase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP- 2-Nluc plasmid.
 
Promoter of glucose-6-phosphate isomerase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP- 2-Nluc plasmid.
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===Usage and Biology===
 
===Usage and Biology===
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===Usage===
 
  
 
By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''E. coli'' was constructed, and we hoped that it could reduce glyoxylic acid in ''E. coli'' to get fluorescence signal in the next processes we design.
 
By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''E. coli'' was constructed, and we hoped that it could reduce glyoxylic acid in ''E. coli'' to get fluorescence signal in the next processes we design.
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:'''Fig 2.''' Gene circuit of GRHPR.
 
:'''Fig 2.''' Gene circuit of GRHPR.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:28, 18 October 2021


pYLXP-2

Promoter of glucose-6-phosphate isomerase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP- 2-Nluc plasmid.

Usage and Biology

By codon optimization and adding a 6His-tag, the sequence suitable for expression in E. coli was constructed, and we hoped that it could reduce glyoxylic acid in E. coli to get fluorescence signal in the next processes we design.

The coding sequence of target gene was inserted into an expression vectors with BBa_K880005(BBa_J23100 & BBa_B0034) to obtain BBa_K3332056. We transformed the constructed plasmid into E. coli BL21 (DE3) to verify its successful heterologous expression.

Fig 2. Gene circuit of GRHPR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 701
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 994
    Illegal BsaI.rc site found at 887
    Illegal SapI.rc site found at 1178