Difference between revisions of "Part:BBa K3893026"

Revision as of 19:36, 15 October 2021

luxI constitutive expression Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part is part of a bigger part to assemble the release system of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, fundamental piece of our population oscilator.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
BBa_K2656019: the K2656013 luxI sequence in its Golden Braid standardized version
Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence C0161adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon optimized for E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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−	Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence[https://parts.igem.org/Part:BBa_C0161 C0161]adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon optimized for E. coli.	+	Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161 C0161]adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon optimized for E. coli.