Difference between revisions of "Part:pSB1AK3"
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Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05) | Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05) | ||
| − | *Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR) | + | *Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR). See also [[Part:BBa_J18907]] (aka VR2) for lower temperature sequencing reactions. |
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| + | *This vector is also available in the Fusion (Freiburg) Biobrick format: [[Part:BBa_J18902]] | ||
Latest revision as of 15:53, 26 February 2009
High copy BioBrick assembly plasmid
pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AK3
Usage and Biology
Kanamycin resistant up to 5000 ug/ml.
Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)
- Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.
- This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18902
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3174 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal XhoI site found at 1035
Illegal XhoI site found at 2061 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3168
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3168
Plasmid lacks a suffix.
Illegal XbaI site found at 3183
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2207