Difference between revisions of "Part:BBa K3740018"
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Modulation of protein expression level | Modulation of protein expression level | ||
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=2021 SZPT-China= | =2021 SZPT-China= | ||
Revision as of 06:29, 10 October 2021
RBS004
Description
Modulation of protein expression level
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
2021 SZPT-China
Biology
Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS004 (BBa_K3740018) using random primers and PCR, and the constructed plasmid J23100-RBS004-sfGFP-rrn B T1 (BBa_K3740059). Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrn B T1 (BBa_K3740058)
Usage
Constructed J23100-RBS004-sfGFP-rrn B T1 (BBa_K3740059) was transformed into E. coli DH5α. we quantified the fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2
Characterization
The average fluorescence intensity of sfGFP induced by RBS004 (BBa_K3740018), was less than 3% of B0034 (BBa_B0034).
