Difference between revisions of "Part:BBa K3484001:Design"
Line 12: Line 12:
 
===Source===
 
===Source===
 
Parts used for this composite are all from previous entries of the iGEM Registry:
 
Parts used for this composite are all from previous entries of the iGEM Registry:
−
** Promoter (<partinfo>BBa_J23100</partinfo>) + Ribosome Binding Site (<partinfo>BBa_B0034</partinfo>): <partinfo>BBa_K880005</partinfo>
+
* Promoter (<partinfo>BBa_J23100</partinfo>) + Ribosome Binding Site (<partinfo>BBa_B0034</partinfo>): <partinfo>BBa_K880005</partinfo>
−
** Protein domain (intein-mediated T3 biosensor): <partinfo>BBa_K3484000</partinfo>]
+
* Protein domain (intein-mediated T3 biosensor): <partinfo>BBa_K3484000</partinfo>]
−
** Double terminator (<partinfo>BBa_B0012</partinfo> -<partinfo>BBa_B0011</partinfo>): <partinfo>BBa_B0014</partinfo>
+
* Double terminator (<partinfo>BBa_B0012</partinfo> -<partinfo>BBa_B0011</partinfo>): <partinfo>BBa_B0014</partinfo>
  
 
===References===
 
===References===
 
Look for references at <partinfo>BBa_K3484000</partinfo>.
 
Look for references at <partinfo>BBa_K3484000</partinfo>.

Revision as of 11:55, 27 October 2020


Intein-mediated T3(THRB) → sfGFP (transcriptional unit)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 235
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 722
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 278
    Illegal SapI.rc site found at 1637


Design Notes

Special considerations have been taken into account for the construction of the protein domain and can be seen at the registry of the basic part BBa_K3484000.


Source

Parts used for this composite are all from previous entries of the iGEM Registry:

References

Look for references at BBa_K3484000.