Difference between revisions of "Part:BBa K3411040"

Revision as of 00:25, 27 October 2020

NarL Gene

Lambert_GA iGEM's NarL system is modeled after E.coli’s natural nitrite and nitrate sensor, the Nar Operon, which includes the NarL response regulator, the gene listed above, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects both nitrite and nitrate. This specific part is the isolated NarL gene.

The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, BBa_K1682018) NarL System serves to accept the phosphate group from NarX. The promoter BBa_J23106, a constitutive Anderson Promoter, initiates the production of TetR. TetR represses the PtetO promoter, producing NarL. In the presence of nitrate, NarX phosphorylates NarL. Therefore, phosphorylated NarL measures nitrate levels. By using the corrected difference, our NarP (Lambert_GA iGEM’s NarP composite part BBa_K3411010) and NarL composite parts can evaluate nitrate levels.

Lambert_GA iGEM will continue cloning and begin characterization of our NarL part in the coming year as a part of our two-year project, AgroSense.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 174
Illegal BglII site found at 342
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3411040 short</partinfo>
-Lambert iGEM's NarL system is modeled after <i>E.coli’s</i> natural nitrite and nitrate sensor: the Nar Operon, which includes the NarL response regulator, the gene listed above, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects both nitrite and nitrate. This specific part is the isolated NarL gene.
+Lambert_GA iGEM's NarL system is modeled after <i>E.coli’s</i> natural nitrite and nitrate sensor, the Nar Operon, which includes the NarL response regulator, the gene listed above, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects both nitrite and nitrate. This specific part is the isolated NarL gene.
-The listed NarL’s role within Lambert iGEM’s (improved from HKUST-Rice 2015,
+The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, [https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018]) NarL System serves to accept the phosphate group from NarX. The promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, initiates the production of TetR. TetR represses the PtetO promoter, producing NarL. In the presence of nitrate, NarX phosphorylates NarL. Therefore, phosphorylated NarL measures nitrate levels. By using the corrected difference, our NarP (Lambert_GA iGEM’s NarP composite part [https://parts.igem.org/Part:BBa_K3411010 BBa_K3411010]) and NarL composite parts can evaluate nitrate levels.
-[https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018]) NarL System. The promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, which initiates the production of TetR. TetR represses the PtetO promoter, producing NarP. In the presence of both nitrate and nitrite, NarQ phosphorylates NarP. Therefore, phosphorylated NarP measures both nitrite and nitrate levels. By using the corrected difference, our NarP and NarL (Lambert iGEM’s improved part of HKUST-Rice 2015 [https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018] composite parts are able to evaluate the nitrite levels.
-Lambert iGEM plans on cloning and characterizing our NarL part in the coming year as a part of our two-year project, AgroSense.
+Lambert_GA iGEM will continue cloning and begin characterization of our NarL part in the coming year as a part of our two-year project, AgroSense.