Difference between revisions of "Part:BBa K3110051:Design"
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===References=== | ===References=== | ||
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| + | <li>Zhang, G., Brokx, S., & Weiner, J. H. (2005). Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nature Biotechnology, 24(1), 100–104. doi:10.1038/nbt1174 </li> | ||
| + | <li>Team ETH Zurich 2015 | ||
| + | </ul> | ||
Revision as of 19:12, 21 October 2019
lldRO1-J23117-lldRO2 Strong RBS YebF-IL12
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 78
Illegal NheI site found at 101 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part contains:
- The Promoter and Operator Region: lldRO1-J23117-lldRO2 (Part:BBa_K1847008)
- A strong RBS: Part:BBa_B0034
- Gene Construct: YebF-IL12-FLAG (Part:BBa_K3110050)
- Double terminator: Part:BBa_I746916
Source
YebF-IL12-FLAG tag was synthesized by Twist Bioscience. lldRO1-J23117-lldRO2 + B0034 and B0015 were obtained as gBlocks from IDT and these 3 parts were joined together using SOEing (Spliced Overlap Extension) PCR.
References
- Zhang, G., Brokx, S., & Weiner, J. H. (2005). Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli. Nature Biotechnology, 24(1), 100–104. doi:10.1038/nbt1174
- Team ETH Zurich 2015