Difference between revisions of "Part:BBa K3110051:Design"

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===Design Notes===
 
===Design Notes===
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Here we have put a lldRO1-J23117-lldRO2 along with B0034 (which is a strong RBs) and downstream to it, we have attached YebF-IL12 and FLAG-tag.
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This part contains:
 +
<ul>
 +
<li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]])
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<li>A strong RBS: [[Part:BBa_B0034]]
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<li>Gene Construct: YebF-IL12-FLAG ([[Part:BBa_I746916]])
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<li>Double terminator: [[Part:BBa_I746916]]
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</ul>
  
 
===Source===
 
===Source===

Revision as of 18:54, 21 October 2019


lldRO1-J23117-lldRO2 Strong RBS YebF-IL12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains:

Source

YebF-IL12-FLAG tag was synthesized by Twist Bioscience. lldRO1-J23117-lldRO2 + B0034 and B0015 were obtained as gBlocks from IDT and these 3 parts were joined together using SOEing (Spliced Overlap Extension) PCR.

References