Difference between revisions of "Part:BBa K2934004:Design"

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linker - [https://parts.igem.org/Part:BBa_K2934005 BBa_K2934005]
 
linker - [https://parts.igem.org/Part:BBa_K2934005 BBa_K2934005]
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The combination was planned and made in our lab using the pBE-S commercial plasmid made by TaKaRa (<em>B. subtilis</em> Secretory Protein Expression System) [1].
 
  
 
===References===
 
===References===
 
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system
 
[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system

Revision as of 16:34, 17 October 2019


AmyE - Glucose Oxidase-Histag for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 487
    Illegal BsaI.rc site found at 433
    Illegal BsaI.rc site found at 1258


Design Notes

Source

GOx - BBa_K2934000

AmyE signal peptide - BBa_K2273023

linker - BBa_K2934005

References

[1] https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/b-subtilis-expression-system