Difference between revisions of "Part:BBa B0030"

Revision as of 10:28, 17 October 2018 (view source) Adrireq (Talk | contribs) (→‎Usage and Biology) ← Older edit		Revision as of 10:29, 17 October 2018 (view source) Adrireq (Talk | contribs) (→‎Usage and Biology) Newer edit →
Line 24:		Line 24:
	</html>		</html>
−	===Usage and Biology===	+	===Contribution===
	Group: Valencia_UPV iGEM 2018		Group: Valencia_UPV iGEM 2018
	<br>		<br>

---

Revision as of 10:29, 17 October 2018

RBS.1 (strong) -- modified from R. Weiss

Strong RBS based on Ron Weiss thesis. Strength is considered relative to BBa_B0031, BBa_B0032, BBa_B0033 and BBa_B0034.

IIT Madras 2016's Characterization

Modelling

Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts modelling]

Experimentation

Biobrick RBSs B0030, B0031, B0032, B0034 were used in our 'Noise in Device' experiment to understand the role of RBS parts in giving rise to noise.

Note: not compatible with R0053 promoter due to likely transcript secondary structure This combination yielded very low gfp expression (see BBa_I7108). [jcb 8/3/05].

Sequence and Features

---

Team Warsaw 2010's measurement

RBS strength (relative to B0034): 91,84%

Contribution

Group: Valencia_UPV iGEM 2018
Author: Adrián Requena Gutiérrez, Carolina Ropero
Summary: We have adapted the part to be able to assemble transcriptional units with the Golden Gate method and we have done the characterization of this RBS.
Documentation:
BBa_K2656009 is the BBa_B0030 RBS standardized into the Golden Braid assembly method. Thus, it is both compatibe with the BioBrick and Golden Gate grammar. It also includes the BioBrick equivalent scar in the 3' extreme, so the insertion of this supplementary bases ensure correct spacing for the CDS expression when assembled into a TU.

Characterization of the this part was performed with the transcriptional unit BBa_K2656101, which was used in two comparative RBS expression experiments with composite parts BBa_K2656102, BBa_K2656103 and BBa_K2656100, BBa_K2656104. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator.

By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model]and rationale choose its optimized values based for each RBS tested.

We have calculated the relative strenght between the different RBS, taking BBa_K2656009 strong RBS as a reference. It has been defined as the quotient between the values of the protein in equilibrium of the results of the simulation of one RBS and the reference RBS. Likewise, a ratio between p parameters of the different RBS parts and p parameter of the reference RBS has been calculated.

Table 2. BBa_K2656008 (GB BBa_B0032 RBS) relative strength and p ratio.
Parameter	Value
Relative strength	1
p parameter ratio (pRBS/pref)	1

Functional Parameters

(Relative to BBa_B0034)