Difference between revisions of "Part:BBa K2623011"
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The sequence encoding for the gene is preceded by a promoter <partinfo>BBa_J23100</partinfo>, an RBS <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K2623004</partinfo>, <partinfo>BBa_K2623005</partinfo>, <partinfo>BBa_K2623006</partinfo> and followed by a double terminator <partinfo>BBa_B0015</partinfo> in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA. | The sequence encoding for the gene is preceded by a promoter <partinfo>BBa_J23100</partinfo>, an RBS <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K2623004</partinfo>, <partinfo>BBa_K2623005</partinfo>, <partinfo>BBa_K2623006</partinfo> and followed by a double terminator <partinfo>BBa_B0015</partinfo> in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA. | ||
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| − | [[Image:PKaiBC RpaA .gif|thumb|700px|Fig.1]] | + | [[Image:PKaiBC RpaA .gif|thumb|700px|Fig.1 Simplified model of the central circadian oscillator and output activities.]] |
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Revision as of 18:15, 16 October 2018
Phosphorylation pathsway builder of Kai
Summary
The sequence encoding for the gene is preceded by a promoter BBa_J23100, an RBS BBa_B0034, BBa_K2623004, BBa_K2623005, BBa_K2623006 and followed by a double terminator BBa_B0015 in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]