Difference between revisions of "Part:BBa K2623011"

Revision as of 02:18, 14 October 2018

Phosphorylation pathsway builder of Kai

Summary

The sequence encoding for the gene is preceded by a promoter BBa_J23100, an RBS BBa_B0034, BBa_K2623004, BBa_K2623005, BBa_K2623006 and followed by a double terminator BBa_B0015 in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA.

Fig.1

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 188
Illegal BglII site found at 3666
Illegal BglII site found at 3735
Illegal BamHI site found at 2248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2623011 short</partinfo>
-===SUMMARY===
+===Summary===
 The sequence encoding for the gene is preceded by a promoter <partinfo>BBa_J23100</partinfo>, an RBS <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K2623004</partinfo>, <partinfo>BBa_K2623005</partinfo>, <partinfo>BBa_K2623006</partinfo> and followed by a double terminator <partinfo>BBa_B0015</partinfo> in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA.
 <br>