Difference between revisions of "Part:BBa K2623016"

Revision as of 14:58, 1 October 2018

Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator)

SUMMARY

TDP circuit is used to express exocrine protein SAHS. We added amilCP BBa_K592009 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100 and an RBS BBa_B0034, and followed by a double terminator BBa_B0015.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 279
Illegal XhoI site found at 503
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 558
Illegal SapI.rc site found at 553

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2623016 short</partinfo>
+===SUMMARY===
 TDP circuit is used to express exocrine protein SAHS.
-In order to facilitate the characterization and purification of SAHS protein, we added the promoter J23100 + RBS + SAHS protein + RBS + K592009 + B0015 to pSB1C3.
+We added amilCP <partinfo>BBa_K592009</partinfo> as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter <partinfo>BBa_J23100</partinfo> and an RBS <partinfo>BBa_B0034</partinfo>, and followed by a double terminator <partinfo>BBa_B0015</partinfo>.
 <!-- Add more about the biology of this part here