Difference between revisions of "Part:BBa I739003"
 
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===Part Structure===
 
===Part Structure===
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<p>The Biobrick encodes LuxR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0062 BBa_C0062]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of luxR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
+
<p>The Biobrick encodes LuxR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0062 BBa_C0062]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23105 BBa_J23105] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of luxR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
  
 
===Mode of Action===
 
===Mode of Action===
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===Purpose===
 
===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. When complexed with HSL, the constitutively synthesized LuxR interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739104 BBa_I739104] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739105 BBa_I739105] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739006 BBa_I739006] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739007 BBa_I739007] respectively.</p>
+
<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. When complexed with HSL, the constitutively synthesized LuxR interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739104 BBa_I739104] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739105 BBa_I739105] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739006 BBa_I739006] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739007 BBa_I739007] respectively.</p>
  
 
===Testing===
 
===Testing===

Latest revision as of 19:51, 26 October 2007

Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)

Part Structure

The Biobrick encodes LuxR (BBa_C0062) under control of the constitutive promoter BBa_J23105 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.

Mode of Action

Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. AHL). This complex binds to a palindromic site on the promoter BBa_R0062, increasing the rate of transcription. So far, this LuxI/R system is the best characterized system of all cell-cell signaling systems.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. When complexed with HSL, the constitutively synthesized LuxR interacts with the double promoters BBa_I739104 and BBa_I739105 which are parts of composites BBa_I739006 and BBa_I739007 respectively.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]