Difference between revisions of "Cell-free chassis/Commercial E. coli S30"
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||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Optimum_Temp <font face=georgia color=#000066 size=4>Optimum temperature</font>] | ||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Optimum_Temp <font face=georgia color=#000066 size=4>Optimum temperature</font>] | ||
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Peak_Time <font face=georgia color=#000066 size=4>Peak time</font>] | ||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Peak_Time <font face=georgia color=#000066 size=4>Peak time</font>] | ||
| − | ||<font face=georgia color=#000066 size=4>Product stability</font> | + | ||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Product_Stability <font face=georgia color=#000066 size=4>Product stability</font>] |
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Expression_Capacity <font face=georgia color=#000066 size=4>Expression capacity</font>] | ||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Expression_Capacity <font face=georgia color=#000066 size=4>Expression capacity</font>] | ||
||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Expression_Lifespan <font face=georgia color=#000066 size=4>Expression lifespan</font>] | ||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Commercial_E.coli_S30/Expression_Lifespan <font face=georgia color=#000066 size=4>Expression lifespan</font>] | ||
Revision as of 12:34, 21 October 2007
Commercial E. coli S30
| Cell-Free Systems | Chassis description | Optimum temperature | Peak time | Product stability | Expression capacity | Expression lifespan |
Description
The Commercial E. coli S30 extract was purchased from Promega. It was prepared by modifications of the method described by Zubay et al (1980). E. coli strain B deficient in OmpT endoproteinase and lon protease activity was used.
The simple constitutive gene expression device BBa_I13522 has been used to characterize this cell-free chassis
References
Please refer to the Promega cell extract user guide for detailed information about the Commercial E. coli S30 extract.