Difference between revisions of "Part:BBa I739005"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I739005 short</partinfo>
 
<partinfo>BBa_I739005 short</partinfo>
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This construct can be used as second cistron expressing a reporter gene in a multicistronic construct.
 
This construct can be used as second cistron expressing a reporter gene in a multicistronic construct.
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===Part Structure===
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<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged eYFP ([https://parts.igem.org/wiki/index.php/Part:BBa_E0032 BBa_E0032]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of tetR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
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===Mode of Action===
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<p>TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses transcription. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.</p>
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===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 1''. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p>
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===Testing===
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<p>Checked for uniqueness of restriction enzyme cleavage sites:<br>
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Eco: ok<br>
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Xba: ok<br>
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Spe: ok<br>
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Pst: ok<br>
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</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:42, 12 October 2007

Promoterless expression cassette for EYFP +LVA (B0034.E0032.B0015)

The Biobrick contains the Ribosome binding site BBa_B0034 upstream of a LVA tagged YFP gene (short halflife). The transcription is terminated by the double terminator BBa_B0015.

This construct can be used as second cistron expressing a reporter gene in a multicistronic construct.

Part Structure

The Biobrick encodes LVA-tagged eYFP (BBa_E0032) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.

Mode of Action

TetR binds to the pTet regulator (BBa_R0040) and represses transcription. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 1. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]