Difference between revisions of "Part:BBa I739003"
Stefan Luzi (Talk | contribs) (→Part Structure) |
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===Mode of Action=== | ===Mode of Action=== | ||
| − | <p> | + | <p>Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter [https://parts.igem.org/wiki/index.php/Part:BBa_R0062 BBa_R0062], increasing the rate of transcription.</p> |
===Purpose=== | ===Purpose=== | ||
Revision as of 12:44, 11 October 2007
Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)
Part Structure
The Biobrick encodes luxR (BBa_C0062) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.
Mode of Action
Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter BBa_R0062, increasing the rate of transcription.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 2. The constitutively synthesized lacI interacts with .... A similar (but not used) construct in this context is BBa_Q04121.
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]