Difference between revisions of "Part:BBa I739001"
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===Purpose=== | ===Purpose=== | ||
| − | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project | + | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 1''. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p> |
===Testing=== | ===Testing=== | ||
Revision as of 12:24, 11 October 2007
Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)
Part Structure
The Biobrick encodes LVA-tagged tetR (BBa_C0040) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.
Mode of Action
TetR binds to the pTet regulator (BBa_R0040) and represses transcription. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 1. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]