Difference between revisions of "Part:BBa I739002"
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<partinfo>BBa_I739002 short</partinfo> | <partinfo>BBa_I739002 short</partinfo> | ||
The Biobrick encodes lacI (BBa_C0012) under control of the constitutive promoter (BBa_J23100) followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015 | The Biobrick encodes lacI (BBa_C0012) under control of the constitutive promoter (BBa_J23100) followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015 | ||
+ | |||
+ | ===Part Structure=== | ||
+ | <p>The Biobrick encodes lacI ([https://parts.igem.org/wiki/index.php/Part:BBa_C0012 BBa_C0012]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of lacI is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p> | ||
+ | |||
+ | ===Mode of Action=== | ||
+ | <p>TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.</p> | ||
+ | |||
+ | ===Purpose=== | ||
+ | <p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed ''Part 1''. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p> | ||
+ | |||
+ | ===Testing=== | ||
+ | <p>Checked for uniqueness of restriction enzyme cleavage sites:<br> | ||
+ | Eco: ok<br> | ||
+ | Xba: ok<br> | ||
+ | Spe: ok<br> | ||
+ | Pst: ok<br> | ||
+ | </p> | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 12:00, 11 October 2007
Constitutive expression cassette for LacI +LVA (J23100.B0034.C0012.B0015)
The Biobrick encodes lacI (BBa_C0012) under control of the constitutive promoter (BBa_J23100) followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015
Part Structure
The Biobrick encodes lacI (BBa_C0012) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015.
Mode of Action
TetR binds to the pTet regulator (BBa_R0040) and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed Part 1. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]