Difference between revisions of "Part:BBa E0840"

Revision as of 20:10, 18 October 2016

GFP generator

BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.

Usage and Biology

See BBa_E0040 for additional details.
BBa_E0840 is often used to quantify the behavior of transcriptional control devices such as promoters.
BBa_E0840 has a strong ribosome binding site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 665

Improvements

Group: TU Delft 2016

Author: Lycka Kamoen and María Vázquez Vitali

Summary: Part BBa_E0840 does not contain any promoter, thus it can only be used if cloned downstream of one. It is also for this reason that this part (BBa_E0840) did not have any characterization. We have improved this BioBrick in 2 different ways: on one hand, we provided 5 ready-to-go expression devices for green fluorescence (with promoters of different strengths), which can be used by future teams; on the other hand, this has allowed us to provide some characterization of this GFP part. Using PCR primers, we added either of the constitutive promoters BBa_J23100, BBa_J23108, BBa_J23105, BBa_J23117 and BBa_J23113 to this part, to create five new biobrick devices. All of these biobricks were characterized by measuring their spectrum and growth cycle.

New parts: BBa_K1890020, BBa_K1890021, BBa_K1890022, BBa_K1890023, BBa_K1890024.

@@ Line 17: / Line 17: @@
 <p><b>Author:</b> Lycka Kamoen and María Vázquez Vitali</p>
 </html>
-<b>Summary:</b> Part <partinfo>BBa_E0840</partinfo> does not contain any promoter, thus it can only be used if cloned downstream of a promoter. It is also for this reason that this part (<partinfo>BBa_E0840</partinfo>) did not have any characterization. We have improved this BioBrick in 2 different ways: on one hand, we provided 5 ready-to-go expression devices for green fluorescence (with promoters of different strengths), which can be used by future teams; on the other hand, this has allowed us to provide some characterization of this GFP part. Using PCR primers, we added either of the constitutive promoters <partinfo>J23100</partinfo>, <partinfo>J23108</partinfo>, <partinfo>J23105</partinfo>, <partinfo>J23117</partinfo> and <partinfo>J23113</partinfo> to this part, to create five new biobrick devices. All of these biobricks were characterized by measuring their spectrum and growth cycle.
+<b>Summary:</b> Part <partinfo>BBa_E0840</partinfo> does not contain any promoter, thus it can only be used if cloned downstream of one. It is also for this reason that this part (<partinfo>BBa_E0840</partinfo>) did not have any characterization. We have improved this BioBrick in 2 different ways: on one hand, we provided 5 ready-to-go expression devices for green fluorescence (with promoters of different strengths), which can be used by future teams; on the other hand, this has allowed us to provide some characterization of this GFP part. Using PCR primers, we added either of the constitutive promoters <partinfo>J23100</partinfo>, <partinfo>J23108</partinfo>, <partinfo>J23105</partinfo>, <partinfo>J23117</partinfo> and <partinfo>J23113</partinfo> to this part, to create five new biobrick devices. All of these biobricks were characterized by measuring their spectrum and growth cycle.
 <b>New parts:</b> <partinfo>K1890020</partinfo>, <partinfo>K1890021</partinfo>, <partinfo>K1890022</partinfo>, <partinfo>K1890023</partinfo>, <partinfo>K1890024</partinfo>.