Difference between revisions of "Part:BBa C0012"
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[[Help:Protein coding|Coding region]] for the LacI protein with an LVA degradation tail and without      an [[Help:Ribosome Binding Site|RBS]]. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)] binds      to LacI and inhibits its operation, therefore promoting transcription.   
 
[[Help:Protein coding|Coding region]] for the LacI protein with an LVA degradation tail and without      an [[Help:Ribosome Binding Site|RBS]]. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)] binds      to LacI and inhibits its operation, therefore promoting transcription.   
  
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A rapid degradation tail ([[Help:Tag|LVA]]) has been added to improve the High to Low      performance of this part.
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A rapid degradation tail ([[Help:Tag|LVA]]) has been added to improve the switch time for High to Low      performance of this part.
  
 
===Usage and Biology===
 
===Usage and Biology===
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This particular LacI protein is derived from e.coli and contributed by Michael Elowitz (see Part Design for more information)
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This particular LacI protein was derived from ''E. coli'' and contributed by Michael Elowitz (See Part Design for more information.)
  
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[[Image:LacRep-black.jpg|frame|left|Image from the Protein Database]]
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[[Image:LacRep-black.jpg|frame|left|Image from the Protein Data Bank (PDB)]]

Revision as of 20:53, 18 May 2007

lacI repressor from E. coli (+LVA)

Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)] binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.

Usage and Biology

This particular LacI protein was derived from E. coli and contributed by Michael Elowitz (See Part Design for more information.)

Image from the Protein Data Bank (PDB)