Difference between revisions of "Part:BBa K1587006:Design"

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===References===
 
===References===
  
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- Levskaya, 2005, Nature. Synthetic biology: engineering E. coli to see light <br>
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- Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442<br>
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- Lee, 2013 PLOS. Switchable gene expression in E. coli using a miniaturized photobioreactor <br>
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- Lee JM, Lee J, Kim T & Lee SK (2013) Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor. PLoS ONE 8: e52382<br>
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- Gabetta, 2001. PNAS. Genetic engineering of phytochrome biosynthesis in bacteria <br>
+
- Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571<br>

Latest revision as of 21:03, 18 September 2015

pompC and lacI box regulated cI expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

We used the sequence BBa_R0082 for pompC, BBa_R0010 for lacI bindig site, BBa_B0030 for the RBS and BBa_K327018 for cI.

References

- Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442
- Lee JM, Lee J, Kim T & Lee SK (2013) Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor. PLoS ONE 8: e52382
- Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571