Difference between revisions of "Part:BBa K1475004:Design"
 
Line 22: Line 22:
  
 
===Source===
 
===Source===
−
Organizm: “Escherichia coli”
+
Organizm: ''Escherichia coli''
  
 
===References===
 
===References===
 
1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677  
 
1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677  
 
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components
 
2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components

Latest revision as of 12:55, 16 October 2014

Cons. promoter, RBS, TetR and a double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed using PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040), the LVA rapid degradation tag was removed in the process.
The PCR was done using the following primers:
Forward primer (contains: XbaI restriction site, Promoter:BBa_J23106 and RBS:BBa_B0030):
CGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCATTAAAGAGGAGAAATACTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAAC
Reverse primer(contains: SpeI restriction site, Terminator:BBa_B1002):
CGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTAGGACCCACTTTCACATTTAAGTTG



Source

Organizm: Escherichia coli

References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677 2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components