Difference between revisions of "Part:BBa K1475004:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1475004 short</partinfo>
 
<partinfo>BBa_K1475004 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part was constructed using PCR from the iGEM registry part TetR+LVA ([https://parts.igem.org/Part:BBa_C0040 Part:BBa_C0040]), the LVA rapid degradation tag was removed in the process.
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<br>
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The PCR was done using the following primers:
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<br>
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Forward primer (contains: XbaI restriction site, [https://parts.igem.org/Part:BBa_J23106 Promoter:BBa_J23106] and [https://parts.igem.org/Part:BBa_B0030 RBS:BBa_B0030]):
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<br>
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CGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCATTAAAGAGGAGAAATACTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAAC
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<br>
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Reverse primer(contains: SpeI restriction site, [https://parts.igem.org/Part:BBa_B1002 Terminator:BBa_B1002]):
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<br>
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CGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTAGGACCCACTTTCACATTTAAGTTG
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===Source===
 
===Source===
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Organizm: “Escherichia coli”
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===References===
 
===References===
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1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677
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2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components

Revision as of 12:26, 16 October 2014

Cons. promoter, RBS, TetR and a double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed using PCR from the iGEM registry part TetR+LVA (Part:BBa_C0040), the LVA rapid degradation tag was removed in the process.
The PCR was done using the following primers:
Forward primer (contains: XbaI restriction site, Promoter:BBa_J23106 and RBS:BBa_B0030):
CGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCATTAAAGAGGAGAAATACTAGATGTCCAGATTAGATAAAAGTAAAGTGATTAAC
Reverse primer(contains: SpeI restriction site, Terminator:BBa_B1002):
CGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTAGGACCCACTTTCACATTTAAGTTG



Source

Organizm: “Escherichia coli”

References

1. C. Krafft, et al.: Interaction of Tet Repressor with Operator DNA and with Tetracycline Studied by Infrared and Raman Spectroscopy. Biophysical Journal, Volume 74, Issue 1, January 1998, Pages 63–71. http://www.sciencedirect.com/science/article/pii/S0006349598777677 2. Tetsystems, 2008: Principles and Components Description. http://www.tetsystems.com/science-technology/principles-components