Difference between revisions of "Part:BBa K1073024"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | During cultivation, the chromoprotein amilGFP is expressed and stored intracellularly. The yellow color of the protein can be seen by naked eye, thus the chromoprotein can be used as a reporter or selection marker to distinguish cells easily. | + | During cultivation, the chromoprotein amilGFP is expressed and stored intracellularly. The yellow color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter or selection marker to distinguish cells easily. |
[[Image:Expressed chromoproteins.png|450px]] | [[Image:Expressed chromoproteins.png|450px]] | ||
Revision as of 12:53, 28 September 2013
Constitutively expressed chromoprotein amilGFP
The device consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.
Usage and Biology
During cultivation, the chromoprotein amilGFP is expressed and stored intracellularly. The yellow color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter or selection marker to distinguish cells easily.
iGEM Team Braunschweig 2013:
Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP , eforRed (BBa_K1073022) and aeBlue (BBa_K1073020).
Right: Cell pellet of E. coli XL1 expressing amilGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]