Difference between revisions of "Part:BBa K1073020"
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During cultivation the chromoprotein aeBlue is expressed and stored intracellular. The blue color of the protein can be seen by naked eye, thus you can use the chromoprotein as a selection marker and seperate cells easily. | During cultivation the chromoprotein aeBlue is expressed and stored intracellular. The blue color of the protein can be seen by naked eye, thus you can use the chromoprotein as a selection marker and seperate cells easily. | ||
| − | [[Image: | + | [[Image:Expressed chromoproteins.png|550px]] |
[[Image:aeBlue_pellet.png|200px]] | [[Image:aeBlue_pellet.png|200px]] | ||
'''iGEM Team Braunschweig 2013:''' | '''iGEM Team Braunschweig 2013:''' | ||
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'''Left:''' Liquid cultures of ''E. coli XL1'' expressing chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>), eforRed (<partinfo>BBa_K1073022</partinfo>) and aeBlue. | '''Left:''' Liquid cultures of ''E. coli XL1'' expressing chromoproteins amilGFP (<partinfo>BBa_K1073024</partinfo>), eforRed (<partinfo>BBa_K1073022</partinfo>) and aeBlue. | ||
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'''Right:''' Cell pellet of ''E. coli XL1'' expressing aeBlue. | '''Right:''' Cell pellet of ''E. coli XL1'' expressing aeBlue. | ||
Revision as of 13:54, 27 September 2013
Constitutively expressed chromoprotein aeBlue
The device consists of a strong promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the chromoprotein aeBlue. This results in a constitutively high expression of aeBlue.
Usage and Biology
During cultivation the chromoprotein aeBlue is expressed and stored intracellular. The blue color of the protein can be seen by naked eye, thus you can use the chromoprotein as a selection marker and seperate cells easily.
iGEM Team Braunschweig 2013:
Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP (BBa_K1073024), eforRed (BBa_K1073022) and aeBlue.
Right: Cell pellet of E. coli XL1 expressing aeBlue.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]