Difference between revisions of "Part:BBa C0012"
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<partinfo>BBa_C0012 short</partinfo> | <partinfo>BBa_C0012 short</partinfo> | ||
| − | Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. | + | [[Help:Protein coding|Coding region]] for the LacI protein with an LVA degradation tail and without an [[Help:Ribosome Binding Site|RBS]]. LacI binds to the pLac regulator <bbpart>BBa_R0010</bbpart> and PLlac01 hybrid regulator <bbpart>BBa_R0011</bbpart> and inhibits transcription. [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)] binds to LacI and inhibits its operation, therefore promoting transcription. |
| − | A rapid degradation tail (LVA) has been added to improve the High to Low performance of this part. | + | A rapid degradation tail ([[Help:Tag|LVA]]) has been added to improve the High to Low performance of this part. |
[[Image:LacRep-black.jpg]] | [[Image:LacRep-black.jpg]] | ||
Revision as of 17:41, 17 July 2006
lacI repressor from E. coli (+LVA)
Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)] binds to LacI and inhibits its operation, therefore promoting transcription.
A rapid degradation tail (LVA) has been added to improve the High to Low performance of this part.
Usage and Biology
This particular LacI protein is derived from e.coli and contributed by Michael Elowitz (see Part Design for more information)