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<partinfo>BBa_J45319 AddReview 4</partinfo>
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<I>UniTN-Trento2013</I>
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This part was resubmitted to the registry in pSB1C3 and characterized under the control of pLac promoter placed inside our wintergreen production device <partinfo>BBa_K1065102</partinfo>.
 
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Latest revision as of 13:24, 24 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J45319

Enzymes generator for catalyzing production of salicylic acid from the cellular metabolite chorismate.

User Reviews

UNIQd8fdd3e43e1cbeee-partinfo-00000000-QINU

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UniTN-Trento2013

This part was resubmitted to the registry in pSB1C3 and characterized under the control of pLac promoter placed inside our wintergreen production device BBa_K1065102.

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Reshma Shetty

BBa_J45319, when used in the wintergreen odor biosynthetic system BBa_J45700, appears to produce some salicylate from the cellular metabolite chorismate.

UNIQd8fdd3e43e1cbeee-partinfo-00000006-QINU

Characterization

Gas chromatography analysis of wintergreen odor biosynthetic system

The wintergreen odor biosynthetic system (BBa_J45700) includes the PchA & PchB enzyme generator (BBa_J45319) under the control of a constitutive promoter (BBa_R0011).

Verification of methyl salicylate production from cellular metabolites by wintergreen biosynthetic system
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.