Difference between revisions of "Part:BBa E1010:Experience"
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<caption><p align="justify"><b>Table 4</b> Shows the fluorescence data over time during photobleaching.</p></caption> | <caption><p align="justify"><b>Table 4</b> Shows the fluorescence data over time during photobleaching.</p></caption> | ||
<tr><td align="center"><b>Time (minutes)</b></td><td align="center"><b>Fluorescence (RFU)</b></td> | <tr><td align="center"><b>Time (minutes)</b></td><td align="center"><b>Fluorescence (RFU)</b></td> | ||
− | <tr><td align="left">0</td><td align="right"> | + | <tr><td align="left">0</td><td align="right">48694</td></tr> |
− | <tr><td align="left">20</td><td align="right"> | + | <tr><td align="left">20</td><td align="right">43083</td></tr> |
− | <tr><td align="left">40</td><td align="right"> | + | <tr><td align="left">40</td><td align="right">36842</td></tr> |
− | <tr><td align="left">60</td><td align="right"> | + | <tr><td align="left">60</td><td align="right">30239</td></tr> |
− | <tr><td align="left">80</td><td align="right"> | + | <tr><td align="left">80</td><td align="right">31281</td></tr> |
− | <tr><td align="left">100</td><td align="right"> | + | <tr><td align="left">100</td><td align="right">25273</td></tr> |
− | <tr><td align="left">120</td><td align="right"> | + | <tr><td align="left">120</td><td align="right">21467</td></tr> |
− | <tr><td align="left">140</td><td align="right"> | + | <tr><td align="left">140</td><td align="right">18081</td></tr> |
− | <tr><td align="left">160</td><td align="right"> | + | <tr><td align="left">160</td><td align="right">15251</td></tr> |
− | <tr><td align="left">180</td><td align="right"> | + | <tr><td align="left">180</td><td align="right">14427</td></tr> |
</table> | </table> | ||
|} | |} |
Revision as of 17:04, 14 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
RANDOM SEQUENCE FOUND WITHIN PART
CGCTGATAGTGCTAGTGTAGATCGC is found after the RFP stop codon and before the BioBricks suffix. Should not affect transcription or translation of RFP, but good to keep note of it especially in analyzing sequencing results. (KP of siGEM)
- Please note that the above sequence is the old "barcode" sequence added to all of the original CDSs in the early BioBrick part collections. I.e., it's not a random sequence. See https://parts.igem.org/cgi/htdocs/barcodes.cgi for more information (D. Endy).
- FURTHER NOTE The Registry is not displaying barcodes on any of the original parts. The presented sequence information is wrong. This is a serious bug in the Registry that need to be fixed (D. Endy). Drew 14:34, 1 November 2010 (UTC)
Applications of BBa_E1010
===User Reviews===
UNIQ028bbd5c90bbcde2-partinfo-00000000-QINU
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Carnegie_Mellon 2013 |
Characterization of the Photostability of mRFP1 XL10 Ultracompetent cells were transformed with BBa_E1010 with BBa_R0010 as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 3. Fluorescence values are shown in Table 4.
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KAIST_iGEM_2012 |
Figure 1. E.coli strain MG1655 expressing BBa_E1010 under control of BBa_K907005 after overnight culture. 3mL culture with M9 media in 14ml round bottom tube(left), and centrifuged cells in eppendorf tube(right). The expression of BBa_1010 is clearly observed with naked eye after overnight culture.
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DTU_igem_2010 |
Characterization of RFP BBa_E1010 Method Results
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Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
Nkessler |
We successfully used this part for a read out system, e.g. in BBa_K389016. Additionally we compared it with a luciferase: BBa_K389004. |
UNIQ028bbd5c90bbcde2-partinfo-0000000F-QINU