Difference between revisions of "Part:BBa K1114100:Design"

(Created page with "__NOTOC__ <partinfo>BBa_K1114100 short</partinfo> <partinfo>BBa_K1114100 SequenceAndFeatures</partinfo> ===Design Notes=== <html> This is the <a href="http://2013.igem.org/...")
 
 
Line 21: Line 21:
  
  
−
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K783055“>BBa_K783055</a>.
+
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055</a>.
  
  

Latest revision as of 22:23, 10 September 2013

MoClo format of a modified version of BBa_B0030 with BC fusion sites.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of BBa_B0030 with BC fusion sites. See the BBa_B0030 page for full information on the part. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.


Source

iGEM Distribution Kit


References