Difference between revisions of "Part:BBa K1041000"
| Line 17: | Line 17: | ||
*<partinfo>BBa_E1010</partinfo>: Red Fluorescent Protein from ''Discosoma striata'' <br> | *<partinfo>BBa_E1010</partinfo>: Red Fluorescent Protein from ''Discosoma striata'' <br> | ||
*<partinfo>BBa_B0015</partinfo>: Double Terminator <br> | *<partinfo>BBa_B0015</partinfo>: Double Terminator <br> | ||
| + | *<partinfo>BBa_B0010</partinfo>: Transcriptional terminator with stem loop <br> | ||
| + | *<partinfo>BBa_B0012</partinfo>: Transcriptional terminator for ''E.coli''RNA polymerase <br> | ||
==Characterisation== | ==Characterisation== | ||
Revision as of 11:27, 24 August 2013
RFP Coding Device
Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]
Composite part of the following BioBricks:
| LacI R0010 | B0034 | mRFP1 E1010 | B0015 | |
 |  |  |  |
- BBa_R0010: Promoter (lacI regulated)
- BBa_B0034: RBS (Elowitz 1999)
- BBa_E1010: Red Fluorescent Protein from Discosoma striata
- BBa_B0015: Double Terminator
- BBa_B0010: Transcriptional terminator with stem loop
- BBa_B0012: Transcriptional terminator for E.coliRNA polymerase
Characterisation
Both parts BBa_K1041000 and BBa_J04450 have colonies that appear red under natural light fig.1. The addition of a NdeI site has not effected the ability for the RFP gene to be transcribed.