Difference between revisions of "Part:BBa K1041000"

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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1041000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1041000 SequenceAndFeatures</partinfo>
 +
 +
'''Composite part of the following BioBricks:'''
 +
<partinfo>BBa_J04450 SpecifiedComponents</partinfo>
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*<partinfo>BBa_R0010</partinfo>: Promoter (lacI regulated) <br>
 +
*<partinfo>BBa_B0034</partinfo>: RBS (Elowitz 1999) <br>
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*<partinfo>BBa_E1010</partinfo>: Red Fluorescent Protein from ''Discosoma striata'' <br>
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*<partinfo>BBa_B0015</partinfo>: Double Terminator <br>
  
 
==Characterisation==
 
==Characterisation==

Revision as of 11:19, 24 August 2013

RFP Coding Device

Mutagenesis was used to add a Nde1 site at the start of the RFP coding region of the biobrick BBa_J04450. This enables a restriction digest with Nde1 to be performed allowing the RFP coding region to be excised from the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 781
    Illegal AgeI site found at 893
  • 1000
    COMPATIBLE WITH RFC[1000]

Composite part of the following BioBricks:

LacI
R0010
B0034		mRFP1
E1010
B0015

Characterisation

Fig 1:(Left) BBa_K1041000 and (right) BBa_J04450 visualized under non-UV lightbox

Both parts BBa_K1041000 and BBa_J04450 have colonies that appear red under natural light fig.1. The addition of a NdeI site has not effected the ability for the RFP gene to be transcribed.