Difference between revisions of "Light"

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=Fluorescent Proteins=
 +
[https://parts.igem.org/Featured_Parts:Fluorescent_proteins Featured Parts],
 +
[http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],
 +
 
 +
 
 +
 
 +
{|cellspacing="0" border="1"
 +
|Name
 +
|Exitation [nm]
 +
|Emmission [nm]
 +
|Quant. yield
 +
|Molar extinct.
 +
|BioBrick RFC10
 +
|BioBrick RFC25
 +
|Reference
 +
|-
 +
|style="background:" |Cerulean
 +
|433
 +
|475
 +
|0.62
 +
|43000
 +
|<partinfo>BBa_E2020</partinfo>
 +
|
 +
|
 +
|-
 +
|style="background:" |GFPmut3b
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|501
 +
|511
 +
|
 +
|
 +
|<partinfo>BBa_E0040</partinfo>
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|
 +
|
 +
|-
 +
|style="background:#EEEE00" |amilGFP
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|503
 +
|512
 +
|0.67
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|75200
 +
|<partinfo>BBa_K592010</partinfo>
 +
|
 +
|[http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
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|-
 +
|style="background:" |mVenus
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|515
 +
|528
 +
|0.57
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|92200
 +
|
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|<Partinfo>BBa_I757008</partinfo>
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|
 +
|-
 +
|style="background:" |mOrange
 +
|548
 +
|562
 +
|0.69
 +
|71000
 +
|<partinfo>BBa_E2050</partinfo>
 +
|
 +
|
 +
|-
 +
|style="background:" |mStrawbery
 +
|574
 +
|596
 +
|0.29
 +
|90000
 +
|<partinfo>BBa_K399001</partinfo>
 +
|
 +
|
 +
|-
 +
|style="background:#8B2252" |mRFP1
 +
|584
 +
|607
 +
|0.25
 +
|50000
 +
|<partinfo>BBa_E1010</partinfo>
 +
|
 +
|
 +
|-
 +
|style="background:" |mCherry
 +
|587
 +
|610
 +
|0.22
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|72000
 +
|<partinfo>BBa_E2060</partinfo>
 +
|
 +
|
 +
|-
 +
|style="background:#FF34B3" |eforRed
 +
|589
 +
|609
 +
|0.16
 +
|111300
 +
|<partinfo>BBa_K592012</partinfo>
 +
|
 +
|[http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
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|-
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|style="background:#66CDAA" |cjBlue
 +
|610
 +
|
 +
|
 +
|
 +
|<partinfo>BBa_K592011</partinfo>
 +
|
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|[http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
 +
|-
 +
|style="background:#473C8B" |aeBlue
 +
|
 +
|
 +
|
 +
|
 +
|<partinfo>BBa_K864401</partinfo>
 +
|
 +
|[http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
 +
|-
 +
|style="background:#551A8B" |amilCP
 +
|588
 +
|NA
 +
|NA
 +
|8760
 +
|<partinfo>BBa_K592009</partinfo>
 +
|
 +
|[http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]
 +
|}
 +
<br>
 +
 
 +
=Luciferases=
 +
{|cellspacing="0" border="1"
 +
|Name
 +
|Organism
 +
|Substrate
 +
|pH optimum
 +
|Amino acids
 +
|Mol. mass [kDa]
 +
|Emmission [nm]
 +
|BioBrick RFC10
 +
|BioBrick RFC25
 +
|Reference
 +
|-
 +
|Photinus/Firefly luciferase
 +
|D-luciferin + ATP
 +
|
 +
|
 +
|550
 +
|60.6
 +
|578
 +
|<partinfo>BBa_I712019</partinfo>
 +
|
 +
|
 +
|-
 +
|Renilla luciferase
 +
|coelenterazine + O<sub>2</sub>
 +
|
 +
|
 +
|311
 +
|36.0
 +
|480
 +
|<partinfo>BBa_J52008</partinfo>
 +
|
 +
|
 +
|-
 +
|Gaussia luciferase
 +
|coelenterazine + O<sub>2</sub>
 +
|
 +
|
 +
|189
 +
|20.3
 +
|480
 +
|<partinfo>BBa_K509005</partinfo>
 +
|
 +
|
 +
|-
 +
|Nano luciferase
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|furimazine
 +
|
 +
|
 +
|171
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|19.1
 +
|460
 +
|[http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]
 +
|
 +
|
 +
|}
 +
 
 +
=Photoproteins=
 +
Aequorin, Obelin

Revision as of 00:19, 7 March 2013

Fluorescent Proteins

Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],


Name Exitation [nm] Emmission [nm] Quant. yield Molar extinct. BioBrick RFC10 BioBrick RFC25 Reference
Cerulean 433 475 0.62 43000 BBa_E2020
GFPmut3b 501 511 BBa_E0040
amilGFP 503 512 0.67 75200 BBa_K592010 [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
mVenus 515 528 0.57 92200 BBa_I757008
mOrange 548 562 0.69 71000 BBa_E2050
mStrawbery 574 596 0.29 90000 BBa_K399001
mRFP1 584 607 0.25 50000 BBa_E1010
mCherry 587 610 0.22 72000 BBa_E2060
eforRed 589 609 0.16 111300 BBa_K592012 [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
cjBlue 610 BBa_K592011 [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
aeBlue BBa_K864401 [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
amilCP 588 NA NA 8760 BBa_K592009 [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]


Luciferases

Name Organism Substrate pH optimum Amino acids Mol. mass [kDa] Emmission [nm] BioBrick RFC10 BioBrick RFC25 Reference
Photinus/Firefly luciferase D-luciferin + ATP 550 60.6 578 BBa_I712019
Renilla luciferase coelenterazine + O2 311 36.0 480 BBa_J52008
Gaussia luciferase coelenterazine + O2 189 20.3 480 BBa_K509005
Nano luciferase furimazine 171 19.1 460 [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]

Photoproteins

Aequorin, Obelin