Difference between revisions of "Part:BBa K907004"
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'''<Part Description>''' | '''<Part Description>''' | ||
<br>This part is derivative of [https://parts.igem.org/Part:BBa_K907002 BBa_K907002], twins with [https://parts.igem.org/Part:BBa_K907005 BBa_K907005] | <br>This part is derivative of [https://parts.igem.org/Part:BBa_K907002 BBa_K907002], twins with [https://parts.igem.org/Part:BBa_K907005 BBa_K907005] | ||
| − | <br>[[Image: KAIST_BBa_K907004.JPG | | + | <br>[[Image: KAIST_BBa_K907004.JPG |center|500px]] |
| − | <br>[[Image: KAIST_BBa_K907004_2.JPG | | + | <br>[[Image: KAIST_BBa_K907004_2.JPG |center|500px]] |
[https://parts.igem.org/Part:BBa_E1010 BBa_E1010](mRFP, reversed) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040](wtGFP) are attached to both ends of [https://parts.igem.org/Part:BBa_K907002 BBa_K907002] by overlapping extension PCR. | [https://parts.igem.org/Part:BBa_E1010 BBa_E1010](mRFP, reversed) and [https://parts.igem.org/Part:BBa_E0040 BBa_E0040](wtGFP) are attached to both ends of [https://parts.igem.org/Part:BBa_K907002 BBa_K907002] by overlapping extension PCR. | ||
This part normally generates GFP(Left two, figure 1) in ''E.coli(strain MG1655)''. | This part normally generates GFP(Left two, figure 1) in ''E.coli(strain MG1655)''. | ||
When [https://parts.igem.org/Part:BBa_K907000 BBa_K907000](Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP(Right two, figure 1). | When [https://parts.igem.org/Part:BBa_K907000 BBa_K907000](Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP(Right two, figure 1). | ||
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'''<Part Demonstration>''' | '''<Part Demonstration>''' | ||
Revision as of 07:53, 26 September 2012
Dual phase protein generator(GFP default). mRFP and GFP
<Part Description>
This part is derivative of BBa_K907002, twins with BBa_K907005
BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached to both ends of BBa_K907002 by overlapping extension PCR. This part normally generates GFP(Left two, figure 1) in E.coli(strain MG1655). When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP(Right two, figure 1).
<Part Demonstration>
Figure 1. BBa_K907004 in MG1655 expressing GFP or mRFP. Left two: Default state. Center: MG1655 wild-type positive control. Right two: Inverted state.
<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907001 - Mycobacterium Phage Bxb1 excisionase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907005 - Dual Phase Protein Generator(mRFP default). mRFP and GFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 756
Illegal NheI site found at 779 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 838
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 703
Illegal AgeI site found at 10
Illegal AgeI site found at 122 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 723
Illegal BsaI.rc site found at 806
Illegal BsaI.rc site found at 1499