Difference between revisions of "Part:BBa K525582:Design"
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<partinfo>BBa_K525582 short</partinfo> | <partinfo>BBa_K525582 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | * Polycistronic expression of FNR and fusion protein of BisdA and BisdB. | |
| + | |||
| + | * Sequence of ''bisdA'' and ''bisdB'' was not entered correctly into the parts.igem. For further information see our notes in [https://parts.igem.org/Part:BBa_K123000:Experience BBa_K123000:Experience] and [https://parts.igem.org/Part:BBa_K123001:Experience BBa_K123001:Experience] | ||
| + | |||
| + | * ''bisdA'' and ''bisdB'' BioBricks were sent to the parts.igem in the Freiburg BioBrick assembly standard (RFC 25) leading to illegal AgeI and NgoMIV restriction sites in this sequence | ||
===Source=== | ===Source=== | ||
| + | * Organism: ''bisdA'' and ''bisdB'' originated in ''Sphingomonas bisphenolicum'' AO1 | ||
| + | |||
| + | * DNA (probably) synthesized and with optimated codon-usage for ''E. coli'' | ||
| + | |||
| + | * FNR originated in ''Escherichia coli'' TOP10 | ||
| + | |||
| + | * Anderson promoter <partinfo>J23110</partinfo>, RBS <partinfo>B0034</partinfo>, BisdA <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> from parts.igem and kit plates, respectively. | ||
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===References=== | ===References=== | ||
| + | Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in ''Sphingomonas bisphenolicum'' strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full ''J Appl Microbiol'' 105(4):1158-1169]. | ||
Latest revision as of 20:17, 28 October 2011
Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB, polycistronic
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1449
Illegal BamHI site found at 2187 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
Illegal NgoMIV site found at 850
Illegal AgeI site found at 812
Illegal AgeI site found at 2440 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 746
Design Notes
- Polycistronic expression of FNR and fusion protein of BisdA and BisdB.
- Sequence of bisdA and bisdB was not entered correctly into the parts.igem. For further information see our notes in BBa_K123000:Experience and BBa_K123001:Experience
- bisdA and bisdB BioBricks were sent to the parts.igem in the Freiburg BioBrick assembly standard (RFC 25) leading to illegal AgeI and NgoMIV restriction sites in this sequence
Source
- Organism: bisdA and bisdB originated in Sphingomonas bisphenolicum AO1
- DNA (probably) synthesized and with optimated codon-usage for E. coli
- FNR originated in Escherichia coli TOP10
- Anderson promoter BBa_J23110, RBS BBa_B0034, BisdA BBa_K123000 and BBa_K123001 from parts.igem and kit plates, respectively.
References
Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].