Difference between revisions of "Part:BBa K525512:Design"
 
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* Sequence of ''bisdA'' and ''bisdB'' was not entered correctly into the parts.igem. For further information see our notes in [https://parts.igem.org/Part:BBa_K123000:Experience BBa_K123000:Experience] and [https://parts.igem.org/Part:BBa_K123001:Experience BBa_K123001:Experience]
 
* Sequence of ''bisdA'' and ''bisdB'' was not entered correctly into the parts.igem. For further information see our notes in [https://parts.igem.org/Part:BBa_K123000:Experience BBa_K123000:Experience] and [https://parts.igem.org/Part:BBa_K123001:Experience BBa_K123001:Experience]
 +
* ''bisdA'' and ''bisdB'' BioBricks were sent to the parts.igem in the Freiburg BioBrick assembly standard (RFC 25) leading to illegal AgeI and NgoMIV restriction sites in this sequence
  
  

Latest revision as of 08:58, 18 September 2011

Polycistronic expression of BisdA and BisdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 896
    Illegal BamHI site found at 1284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
    Illegal NgoMIV site found at 421
    Illegal AgeI site found at 383
    Illegal AgeI site found at 1693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 256
    Illegal BsaI site found at 292
    Illegal BsaI site found at 1503


Design Notes

  • Polycistronic expression of BisdA and BisdB.
  • Sequence of bisdA and bisdB was not entered correctly into the parts.igem. For further information see our notes in BBa_K123000:Experience and BBa_K123001:Experience
  • bisdA and bisdB BioBricks were sent to the parts.igem in the Freiburg BioBrick assembly standard (RFC 25) leading to illegal AgeI and NgoMIV restriction sites in this sequence


Source

  • Organism: bisdA and bisdB originated in Sphingomonas bisphenolicum AO1
  • DNA (probably) synthesized and with optimated codon-usage for E. coli
  • All subparts can be found on one of the kit plates


References

Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].